منابع مشابه
Catalysis of protein folding by an immobilized small-molecule dithiol.
The isomerization of non-native disulfide bonds often limits the rate of protein folding. Small-molecule dithiols can catalyze this process. Here, a symmetric trithiol, tris(2-mercaptoacetamidoethyl)amine, is designed on the basis of criteria known to be important for efficient catalysis of oxidative protein folding. The trithiol is synthesized and attached to two distinct solid supports via on...
متن کاملSmall-molecule Catalysis of Native Disulfide Bond Formation in Proteins
Native disulfide bond formation is essential for the folding of many proteins. The enzyme protein disulfide isomerase (POI) catalyzes native disulfide bond formation using a Cys-Gly-HisCys active site. The active-site properties of POI (thiol pK. = 6.7 and disulfide E;O' = -180 mY) are critical for efficient catalysis. This Dissertation describes the design, synthesis, and characterization of s...
متن کاملSingle-molecule fluorescence spectroscopy in (bio)catalysis.
The ever-improving time and space resolution and molecular detection sensitivity of fluorescence microscopy offer unique opportunities to deepen our insights into the function of chemical and biological catalysts. Because single-molecule microscopy allows for counting the turnover events one by one, one can map the distribution of the catalytic activities of different sites in solid heterogeneo...
متن کاملCatalysis of protein folding by protein disulfide isomerase and small-molecule mimics.
Protein disulfide isomerase (PDI) catalyzes the formation of native disulfide pairings in secretory proteins. The ability of PDI to act as a disulfide isomerase makes it an essential enzyme in eukaryotes. PDI also fulfills other important roles. Recent studies have emphasized the importance of PDI as an oxidant in the endoplasmic reticulum. Intriguing questions remain regarding how PDI is able ...
متن کاملAttractive noncovalent interactions in asymmetric catalysis: links between enzymes and small molecule catalysts.
Catalysis by neutral, organic, small molecules capable of binding and activating substrates solely via noncovalent interactions--particularly H-bonding--has emerged as an important approach in organocatalysis. The mechanisms by which such small molecule catalysts induce high enantioselectivity may be quite different from those used by catalysts that rely on covalent interactions with substrates...
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ژورنال
عنوان ژورنال: Nature
سال: 2008
ISSN: 0028-0836,1476-4687
DOI: 10.1038/455303a